The public discussion of a master’s student (Sawsan Abdul Hameed Jassim), Department of Biology - College of Science - University of Anbar, took place on Sunday 23/10/2022 at Jaber Bin Hayyan Hall in the College of Science about her thesis tagged:

)) Genetic profile of Integrons in Extended Spectrum β lactamase-producing Klebsiella pneumonia isolated from Clinical sources in Ramadi City))

This study aimed to analyse the frequency of occurrence of integrons and also study the association between integron carriage and antimicrobial susceptibility for Klebsiella pneumonia in Ramadi city-Anbar. Our results showed that  occurrence of bla CTX-M gene, blaTEM gene and bla SHV gene in K. pneumoniae  was 100%.Among the total 30 isolates of K. pneumoniae, 30(100%) and 9 (30%) were classified as class 1 (intI1) and 2 (intI2) integrons, respectively. Two isolates carried both class 3 , and 2(6.66%) harbored class 1,2, and 3 integrons. , class 1 integrons with identical cassette array were detected in most isolates .The association between integron carriage and antibiotic resistance was most significant for Ampicillin , Pip-Tazo , Ceftriaxon , cefotaxime, and Ceftazidime (P< 0.0001), and also followed by meropenem, and imipenem (P< 0.0001).

According to GC- Mass Analysis of neem extract , it was observed that presence of phytol 61.96%, coumarin 1.02%, hexadecanoic acid 4.40%, cis-13-octadecenoic acid 1.38%, oleic acid 0.84% and thiocarbamic acid 1.37%were the major components in the extract. Results demonstrated that neem leaves extract was quite effective in inhibiting ESBLS activity at P- value : . The  statistical  tests showed that extract  of neem has a good   activity   against   biofilm   production   in K.   pneumoniae with significant   differences   (p< 0.01). Neem extract   exhibited synergistic effect  when  combination  by  checkerboard  technique  with cefotaxime and ceftriaxone  against ESBLs producing-  K. pneumoniae.